Amputations of penis are rare but occasionally necessary for the resection of squamous cell carcinoma. 1-3 Wedge resections of glans penis and glansectomy specimens may also be received. 2 Small biopsies specimens are included in GU small biopsy instructions.

Record the patient identifying information and any clinical information supplied together with the specimen description as designated on the container. See overview page for more detail on identification principles.

Fresh tissue received
  • No
    • Non-routine fixation (not formalin), describe.
  • Yes
    • Special studies required, describe.
    • Ensure samples are taken prior to fixation.
Intraoperative consultation
  • Not performed
  • Performed, describe type and result
    • Frozen section
    • Imprints
    • Other, describe

See general information for more detail on specimen handling procedures.

Inspect the specimen and dictate a macroscopic description.

External InspectionBack to top

Orientate and identify anatomical features of the specimen (glans, coronal sulcus, foreskin, corpus spongiosum, corpora cavernosa, penile urethra). Orientate and designate margins.

Orientation markers

Record additional orientation or designation provided by operating clinician:

  • Absent
  • Present
    • Method of designation (e.g. suture, incision)
    • Featured denoted

Photograph the intact specimen.

Describe the following features of the specimen: 1-4


​Record as stated by the clinician.
  • Biopsy; punch, incisional or excisional biopsy see GU small biopsy instructions
    • Site(s)
  • Foreskin/circumcision
  • Glans, wedge resection
  • Glansectomy
  • Penectomy
    • Partial
    • Total

Anatomical components included (more than one may apply) and specimen size

  • Entire specimen, in three dimensions

Describe and measure components (as applicable) in three dimensions:

  • Glans
  • Shaft
  • Foreskin
  • Coronal sulcus
  • Scrotal skin
  • Testicles

Specimen integrity

  • Intact
  • Disrupted, describe

Photograph the intact specimen.

DissectionBack to top


Paint all relevant surgical margin(s) with ink and record the colours applied.

Section longitudinally along the skin surface.

One cassette of representative with representative sections will suffice if no lesions have been identified.

If suspicious lesions are present or clinical history indicates, submission of the entire specimen may be appropriate.

Wedge resection of glans penis

Paint all relevant surgical margins with ink and record the colours applied.

Serially section in the transverse plane at 3mm intervals. Often submission of the entire specimen may be appropriate.


These specimens usually include the corona, skin margins, urethral meatus and occasionally the tunica.

Paint all relevant surgical margin(s) with ink. Serially section parallel to the urethra (parasagitally) to demonstrate the relationship of tumour to urethra and skin ventrally and dorsally.

Take coronal sections of left and right sides to demonstrate the lateral skin limits.

Submit all sections of entire specimen.


Paint all relevant surgical margin(s), including the proximal urethra, with ink and record the colours applied.

Open the specimen longitudinally along the centre of the ventral surface bisecting the urethra and dividing the specimen into right and left sections.

Section parasagitally along the entire specimen. The urethra should be closely examined for evidence of tumour involvement as this will upstage the tumour.

After opening the specimen may require longer fixation in larger quantity of formalin.

Take longitudinal section(s) to demonstrate urethral, spongiosus, cavernosal and skin margins.

Internal InspectionBack to top

Describe the cut surface appearance including the following items: 1-4


  • Absent
  • Present
    • Number; if more than one tumour, designate and describe each tumour separately

Tumour location(s)

  • Foreskin
    • Mucosal surface
    • Skin surface
  • Sulcus
  • Glans
  • Skin of shaft
  • Penile urethra

Tumour size (mm)

  • Maximum dimension
  • Other dimension

Tumour appearance

  • Nodule
  • Endophytic
  • Exophytic
  • Polyp
  • Papillary
  • Verruciform
  • Plaque
  • Ulcer
  • Pigmented macule, describe
    • Irregular edge
    • Circumscribed edge
    • Uniform pigmentation
    • Variegated pigmentation


  • Absent
  • Present
    • Maximum depth (mm)
  • Lamina propria
  • Corpus spongiosum
  • Tunica albuginea
  • Corpora cavernosa
  • Urethral meatus/urethra

Surgical margins

  • Distance of tumour to the closest margin (mm)
  • Specify margin

Non-tumour lesion

  • Describe
Lymph nodes

Retrieved from resection specimen

  • Describe site(s)
  • Number retrieved

Separately submitted

  • For each specimen container, record specimen number and designation
  • Collective size of tissue in three dimensions (mm)
  • Number of grossly identified lymph nodes submitted
  • Maximum diameter of each (mm)

Photograph the dissected specimen.

ProcessingBack to top

Dissect the specimen further and submit sections for processing as follows: 1-4

Foreskin (circumcision)
  • Submit representative sections if no suspicious lesions are present.
  • If suspicious lesions are present, submit all sections in sequential order.
  • If orientated, submit shave sections from margins in separately designated cassettes.
Wedge resection of glans penis
  • Submit all sections in sequential order.
  • If orientated, submit shave sections from the margin(s) in separately designated cassettes.
  • Submit all sections in sequential order.
  • If orientated, submit shave sections from the margin(s) in separately designated cassettes.

Submit representative sections of:

  • Surgical margins, at least two cassettes of proximal margin including skin, corpora and urethra (more if tumour is close, in which case longitudinal sections may be used)
  • Tumour and adjacent tissue demonstrating deepest level of invasion, 3-4 blocks
  • Longitudinal section through glans and urethra to demonstrate any urethral involvement
  • Uninvolved skin

Submit all lymph nodes

Record details of each cassette.

An illustrated block key similar to those provided below may be useful.

Block allocation keys

Cassette id
No. of pieces
Margins (if applicable)
Glansectomy/wedge resection of glans penis
Cassette id
No. of pieces
Glans penis
Cassette id
No. of pieces
Surgical margins, including skin, corpora and urethra
Tumour and adjacent tissue including deepest level of invasion and tumour closest to the urethra
Skin and corpus cavernosum
Urethral meatus (further)


A/Prof Hemamali Samaratunga for her contribution in reviewing and editing this protocol.

ReferencesBack to top

  1. Lester SC. Manual of Surgical Pathology, Saunders Elsevier, Philadelphia, 2010.
  2. Harnden P, Corbishley C and Freeman A. Dataset for penile cancer histopathology reports, The Royal College of Pathologists, London, 2006.
  3. Mikuz G, Winstanley AM, Schulman CC, Debruyne FM and Parkinson CM. Handling and pathology reporting of circumcision and penectomy specimens. Eur Urol 2004;46(4):434-439.
  4. O’Rourke D, Turner G and Allen D. Tissue Pathways for Urological Pathology, The Royal College of Pathologists, London, 2010.