Cell immunophenotyping

Keywords: Flow cytometry

Specimen:

5 mL blood or 2 mL bone marrow in lithium heparin, ACD or  EDTA.; CSF and other fluids in sterile container; lymph nodes and other tissues in sterile tissue culture medium. See Immunohistochemistry.

The specific ​indication for the test must be specified to allow appropriate testing to be performed. Consider discussion with pathologist prior to requesting test.

Method:

Cell identification using antibodies to cellular antigens (including T and B lymphoid, myeloid, platelet, natural killer, stem cell, epithelial and other non-haemopoietic cells) by flow cytometry or immunoenzymatic techniques.

Application:

Assessment of aetiology of unexplained lymphocytosis;
assessment and classification of  B cell Lymphoproliferative disorders;
classification of acute and chronic leukaemias (see Acute leukaemia, Chronic leukaemia);
detection of residual disease in leukaemia;
assessment and monitoring of stem cell numbers for collection by cytapheresis for peripheral blood progenitor cell transplantation;
assessment of fetomaternal haemorrhage (see Feto-maternal haemorrhage investigation);
lymphocyte subsets (quantitation of CD4 count in eg, HIV infection);
diagnosis of Hereditary spherocytosis; and
diagnosis of Paroxysmal nocturnal haemoglobinuria (PNH).

Interpretation:

The report will include identification of the cell types present, and an interpretative comment from the pathologist. Findings are best interpreted in conjunction with results of other related tests (eg, Blood film morphology, histology, etc.)

See also Lymphocyte immunophenotyping.

Reference:

NCCLS. Clinical Applications of Flow Cytomety; Approved Guideline.Vol 18. NCCLS 1998.

Bain et al. Dacie and Lewis Practical Haematology. Eleventh edition. 2012.