Cell immunotyping

Specimen:

5-10 mL blood or 1-2 mL bone marrow aspirate in lithium heparin or ACD tube; CSF and other fluids in sterile container; lymph nodes and other tissues in sterile tissue culture medium. See Immunohistochemistry.

Method:

Cell identification using antibodies to cellular antigens (including T and B lymphoid, myeloid, platelet, natural killer, stem cell, epithelial and other non-haemopoietic cells) by flow cytometry or immunoenzymatic techniques.

Application:

Assessment of lineage and clonality of B cell Lymphoproliferative disorders;
classification of acute and chronic leukaemias (Acute leukaemia, Chronic leukaemia);
occasionally useful to detect residual disease in leukaemia;
identification of undifferentiated tumours;
investigation of aetiology of unexplained lymphocytosis;
detection of metastatic disease in bone marrow or other tissues; and
assessment and monitoring of stem cell numbers for collection by cytapheresis for peripheral blood progenitor cell transplantation.

Interpretation:

The report will include identification of the cell types present, and an interpretative comment from the pathologist.

See also Lymphocyte immunophenotyping.

Reference:

Chanarin I ed. Laboratory Haematology. Churchill Livingstone 1989.