Chlamydia trachomatis nucleic acid

Keywords: Chlamydia PCR, C trachomatis PCR, C trachomatis NAT, C trachomatis DNA, Chlamydia trachomatis detection

Specimen:

Genital swab, conjunctival swab, endotracheal aspirate (neonates), placed in transport medium and/or smeared directly onto slide.

For nucleic acid probe after amplification, consult laboratory. Some assays use a dry swab while for others the swab must be discarded after special transport medium has been inoculated.

Initial urine specimens are an alternative to genital swabs for both males and females.

Method:

Detection of Chlamydia trachomatis by culture, nucleic acid probe after amplification.

Application:

Urethritis, cervicitis; conjunctivitis.

Pneumonia in the first 6 months of life.

Interpretation:

Culture and nucleic acid probe (after amplification) are more sensitive techniques than DFA and EIA.

Nucleic acid probe after amplification has replaced DFA and EIA and can be performed on initial (not mid-stream) urine specimens as an alternative to collection of a genital swab.

For test-of-cure in cases of genital infection, specimens should not be collected until at least three weeks after completion of antibiotic treatment. An initial urine specimen is adequate for this purpose.

Reference:

Black CM. Clin Microbial Rev 1997; 10: 160-184.