Collagen binding assay

Specimen:

4.5 mL blood added to 0.5 mL citrate.

Method:

Immunoassay (EIA; ELISA) with measure of binding of VWFAg to collagen-coated microtitre plates. Abbreviated VWF:CB.

Reference Interval:

Refer to the laboratory.  Generally 50-200% of the level in pooled normal plasma (guide only).

Application:

Additional functional VWF complementary to the ristocetin cofactor assay in the investigation of a possible bleeding disorder: diagnosis of von Willebrand disease and its variant forms.

See von Willebrand factor (Ristocetin cofactor, VWF:RCo) and von Willebrand factor multimer.

Interpretation:

Generally performed in conjunction with other tests and not performed or interpreted in isolation.

See also von Willebrand disease testing; von Willebrand factor Ag and Ristocetin cofactor.

Reference:

Favaloro EJ. Clin Haematol 2001; 14: 299-319.

Favaloro EJ. Semin Thromb Hemost 2002; 28: 191-202.

Favaloro EJ. Haemophilia 2002; 8: 828-831.