Cytochemistry

Specimen:

Bone marrow aspirate and/or 5 mL blood in EDTA.

Method:

Appropriate cytochemical methods are selected by the pathologist on the basis of the haematological and clinical features.

Specific cytochemical methods available include Acid phosphatase (± tartaric acid), specific and non-specific esterases, periodic acid-Schiff (PAS), Sudan black, myeloperoxidase, lysozyme.

Application:

Rarely used currently. Use and type determined by pathologist to assist diagnosis of acute leukaemias and lymphoproliferative disorders when indicated.

Interpretation:

An interpretative comment usually provided by the pathologist within the overall report. 

See Cell immunophenotyping.

Reference:

Scott CS ed. Leukaemia Cytochemistry - Principles and Practice. Horwood Ltd 1989.