DNA ploidy

Specimen:

10 mL blood or 1-2 mL bone marrow aspirate in lithium heparin; fresh tissue.

Method:

Flow cytometry, image analysis.

Application:

To detect abnormal DNA content (aneuploidy) in neoplastic disorders.

Also useful in the diagnosis of triploidy in an aborted fetus.

Interpretation:

Neoplastic cells may have chromosome alterations which result in an increase or decrease in the total DNA content.

These changes may be of prognostic significance, eg, in ovarian, prostatic and bladder carcinoma.

Consult pathologist.

Reference:

Herrington CS, McGee JO. In: McGee JO et al eds. Oxford Textbook of Pathology. Oxford University Press, 1992.