Direct antiglobulin test

Keywords: DAT, Coombs direct, Direct coombs test

Specimen:

5 mL blood in EDTA tube.

Method:

Polyspecific anti-human globulin serum added to patient’s washed red cells. The test is positive if agglutination is observed.

Application:

Investigation of possible immune haemolysis.

Detection of antibody and/or complement on red cell surface.

Reference:

Ford D. Aust J Med Lab Sci. 1995; 16: 67-70.