Donath-Landsteiner Ab

Keywords: Donath-Landsteiner antibody

Specimen:

Collection by laboratory staff into 2 plain tubes, one at 37°C and one at 4°C.

Method:

Supernatant serum examined for evidence of red cell lysis, comparing a tube incubated at 4°C then 37°C with a tube maintained at 37°C.

Reference Interval:

No lysis in either tube.

Application:

Diagnosis of paroxysmal cold haemoglobinuria.

Interpretation:

The Donath-Landsteiner antibody is an IgG autoantibody that binds to red cells in the cold and fixes complement; lysis occurs when cells are warmed to 37°C.

Presence of the antibody is diagnostic for paroxysmal cold haemoglobinuria. This rare disorder may occur in syphilis and after viral infections.

Reference:

Bain et al. Dacie and Lewis Practical Haematology. Eleventh edition. 2012.