Haemoglobin M

Keywords: HbM

Specimen:

5 mL blood in any anticoagulant.

Method:

Spectrophotometry and electrophoresis on a lysate of washed red cells.

See Haemoglobin electrophoresis, Haemoximetry, Methaemoglobin and Sulphaemoglobin.

Application:

Investigation of chronic unexplained cyanosis.

Suspicion of hereditary methaemoglobinaemia.

Interpretation:

Methaemoglobinaemia due to inherited haemoglobinopathy is defined by the presence of haemoglobin M.

Reference:

Bain et al. Dacie and Lewis Practical Haematology.  11th Ed. 2012.

Blain B. Haemoglobinopathy Diagnosis. 2001. Blackwell Science.