Haemoglobin electrophoresis

Keywords: Haemoglobin EPP, Haemoglobin EPG, Hb EPP, Hb EPG, Haemoglobinopathy screen

Specimen:

5 mL blood in any anticoagulant.

Method:

Electrophoresis of a lysate of washed red cells; haemoglobins identified by mobility.

Reference Interval:

Refer to laboratory. Approximate ranges given below.

Normal adult: mainly HbA, small amounts HbA2 (1.5-3.5%), HbF (<1%).

Application:

Possible Thalassaemia or Haemoglobinopathy, including those haemoglobinopathies associated with DAT negative haemolysis, erythrocytosis, or cyanosis.

Interpretation:

Haemoglobin variants with abnormal electrophoretic mobility will be detected eg, HbS, HbE. 

An interpretative comment will be provided by the pathologist.

Reference:

Working Party of the General Haematology Task Force of the British Committee for Standards in Haematology. Br J Haematol 1998; 101: 783-792.

Bain et al. Dacie and Lewis Practical Haematology. 11th ed. 2012.

Ryan K et al. Significant haemoglobinopathies: guidelines for screening and diagnosis. BJH. 2010; 149; 35-49.