Haemoglobin urine

Specimen:

Freshly voided urine collected without preservative.

Method:

Spectroscopic examination or urinalysis to detect haem pigment.

Urine microscopy to exclude haematuria.

EPG or IFE to distinguish haemoglobin from myoglobin.

Reference Interval:

Not detected in normal urine.

Application:

History of red or dark urine; findings on FBC and blood film suggesting intravascular haemolysis; investigation of immediate haemolytic reaction during Blood transfusion.

See also Myoglobin urine.

Interpretation:

Haemoglobinuria indicates intravascular haemolysis;

release of haemoglobin in amounts exceeding the binding capacity of haptoglobins; and

consequent glomerular filtration of haemoglobin and presence in urine.

Reference:

Bain et al. Dacie and Lewis Practical Haematology. 11th Ed. 2012.