Neutrophil Ab

Keywords: Neutrophil antibody

Description:

Rarely used and only performed in specialised laboratories.

Specimen:

10 mL blood in EDTA for 'direct' tests of neutrophil-bound antibody and 10 mL in plain tube for 'indirect' tests of antibody in serum

Method:

Direct and indirect Immunofluorescence, Flow cytometry, agglutination.

Application:

Investigation of neutropenia, when immune-mediated (including drug-induced) neutropenia is suspected. Investigation of suspected cases of neonatal alloimmune neutropenia (NAIN). Investigation of suspected cases of transfusion-related acute lung injury (TRALI).

Interpretation:

A positive result is seen in immune neutropenia and drug-induced neutropenia eg, secondary to penicillins, cephalosporins, vancomycin.

Antibodies to Human Neutrophil Antigens (HNAs) are seen in cases of NAIN, and TRALI. Results are usually correlated with HNA genotyping results – specialised testing and consultation with pathologist required.

Reference:

Bain et al. Dacie and Lewis Practical Haematology. 11th Ed. 2012.

Muschter S et al. Curr Opin Hematol. 2011; 18, 452-460.

Clay ME et al. Immunohematology. 2010; 26(10, 11-22.

Stroncek D. Vox Sang. 2004; 87 (suppl 1), s91-s94.