Neutrophil function studies

Description:

Uncommonly used and only performed in specialised laboratories with prior arrangement.

Specimen:

10 - 30 mL heparinised blood (consult laboratory).

Method:

Several methods including chemiluminescence, nitroblue tetrazolium, dihydrorhodamine.

Application:

Test indicated for:

Investigation of recurrent pyogenic or fungal infections especially chest, skin and gingival infections.

Initial screening for the diagnosis of chronic granulomatous disease(CGD) and other primary defects in neutrophil function.

Detection of CGD carriers.

Interpretation:

Impairment of phagocytosis indicates impairment of oxidative burst which may indicate CGD or even rarer neutrophil disorder which may be primary or secondary to ongoing pyogenic infection.

Specialised interpretation is required.

Reference:

Coates TD et al. In: Rose NR et al eds. Manual of Clinical Laboratory Immunology. 4th ed.1992. ASM Press.