Oesophageal brushing cytology

Specimen:

Directional reusable brush samples may be collected and rolled directly onto the surface of glass slides for immediate fixation in alcohol or, less commonly, air-drying and post-fixation in methanol. 

Disposable brush sample tips may be cut off into thin layer preparation solution if this technique is available. 

Brush samples should be taken prior to biopsies to reduce blood contamination.

Application:

Investigation of endoscopically identified inflammatory conditions, epithelial tumours and non-epithelial tumours.

Interpretation:

Comments provided by a pathologist on the presence or absence of malignancy.

Highly sensitive technique for identifying Candida infection. 

May also reliably identify Herpes simplex ulceration and Cytomegalovirus infection.

Reference:

Gray W and Kocjan G eds. Diagnostic Cytopathology.  3rd ed. 2010. Churchill Livingstone.