Reptilase time

Specimen:

4.5 mL blood added to 0.5 mL citrate.

Method:

Reptilase is derived from the venom of Bothrops atrox, and clots fibrinogen by a mechanism different from that of thrombin.

Reference Interval:

Consult laboratory. Typically < 20 seconds.

Application:

Investigation of patients with a prolonged Thrombin time (clotting).

Interpretation:

Assists in differentiating the effect of heparin, presence of FDP / D Dimer and dysfibrinogenaemia. A prolonged Thrombin time result will correct to normal using reptilase if this is due to heparin.

Reference:

Bain et al. Dacie and Lewis Practical Haematology.11th Ed. 2012.