Unstable haemoglobin screening test

Specimen:

5 mL blood in any anticoagulant.

Method:

Haemolysate made from washed red cells and examined for the presence of precipitate after incubation with isopropanol.

Reference Interval:

Not detected.

Application:

Investigation of DAT negative haemolytic anaemia where unstable Haemoglobinopathy is suspected.

Interpretation:

Patients with an unstable Haemoglobinopathy may have a chronic or intermittent haemolytic anaemia, or may have increased susceptibility to the haemolytic effects of oxidant drugs. See Heinz body.

Reference:

Winterbourn C. Semin Haematol 1990; 27: 41-50.