von Willebrand factor multimer

Specimen:

4.5 mL blood added to 0.5 mL citrate or 5 mL blood in EDTA.

Method:

Crossed immunoelectrophoresis; SDS-agarose electrophoresis with autoradiography or enzyme-linked antibody staining.

Application:

Characterisation of patients with definite or highly probable von Willebrand's disease into subtypes (that is, 1, 2, 3 or platelet-type VWD).

May also be useful when assessing VWD defects of genetic significance and as a guide to management.

Multimers are not used as a screening test for von Willebrand's disease.

Interpretation:

See von Willebrand disease testing, von Willebrand factor Ag, Ristocetin cofactor and Collagen binding assay.

Reference:

Favaloro EJ. Clin Haematol 2001; 14: 299-319.

Sadler JE. Thromb Haemost 1994: 71; 520-525.