Sample collection and fixation
Cervical samples for LBC are collected using the manufacturer’s instructions and transferred into a liquid based medium which provides immediate fixation and preservation of cellular details.
The entire cervical sample is transferred to the SurePathTM preservative fluid; the head of the collection device is left in the vial
The cervical sample is rinsed from the collection device(s) into the ThinPrep PreservCyt® preservative fluid
Slide preparation and staining
LBC technology is designed to provide a superior slide preparation compared with the conventional Pap smear. Obscuring blood and inflammation are reduced or removed and cellular elements are evenly spread in a circumscribed area. ThinPrepTM and SurePathTM slides may be stained by the standard Papanicolau method or by the suppliers’ proprietary stains.
SurePathTM slides are prepared and stained on the BDPrepStainTM using a gradient density technique and on-board staining
ThinPrep® slides are prepared using the TP 2000 or TP 5000 Processor® using a membrane filtration process. Staining is by conventional Papanicolaou stain or, if intended for review using the ThinPrep Imager®, by the ThinPrep Stain®
Macroscopic appearance of smear
LBC slides present cellular material in an evenly spread, well-circumscribed circular area.
Cellular material is presented in a 13mm diameter circle
Cellular material is presented in a 20mm diameter circle
LBC slides are designed to accommodate both conventional, manual screening and also semi-automated screening.
SurePath™ slides may be screened manually or with assistance of the FocalPointTM
ThinPrep® slides may be screened manually or with assistance of the ThinPrep Imager®