Donath-Landsteiner Ab

Keywords: Donath-Landsteiner antibody


Collection by laboratory staff into 2 plain tubes, one at 37°C and one at 4°C.


Supernatant serum examined for evidence of red cell lysis, comparing a tube incubated at 4°C then 37°C with a tube maintained at 37°C.

Reference Interval:

No lysis in either tube.


Diagnosis of paroxysmal cold haemoglobinuria.


The Donath-Landsteiner antibody is an IgG autoantibody that binds to red cells in the cold and fixes complement; lysis occurs when cells are warmed to 37°C.

Presence of the antibody is diagnostic for paroxysmal cold haemoglobinuria. This rare disorder may occur in syphilis and after viral infections.


Bain et al. Dacie and Lewis Practical Haematology. Eleventh edition. 2012.