The main purpose of this Manual is to provide useful guidelines for the selection of pathology tests and to facilitate interpretation of results.
Contains a comprehensive listing of all genes from the Human Gene Nomenclature Committee (HGNC) database alongside laboratories and tests available in the country.
A manual for the process of macroscopic dissection in Anatomical Pathology laboratories.
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Keywords: Flow cytometry
5 mL blood or 2 mL bone marrow in lithium heparin, ACD or EDTA.; CSF and other fluids in sterile container; lymph nodes and other tissues in sterile tissue culture medium. See Immunohistochemistry.
The specific indication for the test must be specified to allow appropriate testing to be performed. Consider discussion with pathologist prior to requesting test.
Cell identification using antibodies to cellular antigens (including T and B lymphoid, myeloid, platelet, natural killer, stem cell, epithelial and other non-haemopoietic cells) by flow cytometry or immunoenzymatic techniques.
Assessment of aetiology of unexplained lymphocytosis;
assessment and classification of B cell Lymphoproliferative disorders;
classification of acute and chronic leukaemias (see Acute leukaemia, Chronic leukaemia);
detection of residual disease in leukaemia;
assessment and monitoring of stem cell numbers for collection by cytapheresis for peripheral blood progenitor cell transplantation;
assessment of fetomaternal haemorrhage (see Feto-maternal haemorrhage investigation);
lymphocyte subsets (quantitation of CD4 count in eg, HIV infection);
diagnosis of Hereditary spherocytosis; and
diagnosis of Paroxysmal nocturnal haemoglobinuria (PNH).
The report will include identification of the cell types present, and an interpretative comment from the pathologist. Findings are best interpreted in conjunction with results of other related tests (eg, Blood film morphology, histology, etc.)
See also Lymphocyte immunophenotyping.
NCCLS. Clinical Applications of Flow Cytomety; Approved Guideline.Vol 18. NCCLS 1998.
Bain et al. Dacie and Lewis Practical Haematology. Eleventh edition. 2012.
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The RCPA is the leading organisation representing Pathologists and Senior Scientists in Australasia.
Its mission is to train and support pathologists and senior scientists and to improve the use of pathology testing to achieve better healthcare.
The annual scientific meeting for the RCPA which covers the scientific disciplines of Anatomical, Chemical, Forensic, Genetic, General, Haematology, Immunopathology and Microbiology.
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