The main purpose of this Manual is to provide useful guidelines for the selection of pathology tests and to facilitate interpretation of results.
Contains a comprehensive listing of all genes from the Human Gene Nomenclature Committee (HGNC) database alongside laboratories and tests available in the country.
A manual for the process of macroscopic dissection in Anatomical Pathology laboratories.
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Keywords: Genital microscopy culture sensitivities, MCS genital, Genital MCS, Urogenital MCS
Cervical swab collected from the endocervical canal during speculum examination;
Combined low vaginal/rectal swab for detection of Group B steptococcus in pregnancy;
Swab of urethral discharge and/or urethral sampling using fine swab.
Smears should be made directly on a clean glass slide.
Unfixed slides should be handled with care and transported to the laboratory in a suitable container.
For detection of Chlamydia trachomatis and Neisseria gonorrhoeae by nucleic acid probe after PCR amplification, consult laboratory. Some assays use a dry swab while for others the swab must be discarded after special transport medium has been inoculated. Initial urine specimens are an alternative to genital swabs for detection of these organisms.
Microscopy of wet film;
Gram stain and direct IF;
Culture including Neisseria gonorrhoeae, Chlamydia trachomatis (specialised laboratories only), Candida albicans, Gardnerella vaginalis, Group B streptococcus (pathogenic in the neonate), Haemophilus influenzae.
Some laboratories also culture for Trichomonas vaginalis.
EIA and/or molecular genetics for Chlamydia trachomatis, Neisseria gonorrhoeae.
Investigation of mucopurulent cervicitis (see Cervical lesion);
Urethral discharge (see Urethritis);
Pelvic inflammatory disease;
Screening for carriage of Group B streptococcus in pregnancy.
Microscopy of wet film: excess white cells suggest presence of infection; motile trichomonads indicate presence of trichomoniasis, yeasts and hyphae suggest candidiasis; 'clue' cells without an excess of white cells suggest Gardnerella vaginalis infection.
Gram stain: the presence and relative numbers of pus cells and epithelial cells influence interpretation; presence of yeasts supports a diagnosis of candidiasis; intra- and extra-cellular Gram-negative diplococci suggest gonorrhoea.
Culture: results must be interpreted in conjunction with clinical information and the results of microscopy of wet film and Gram stain.
Nucleic acid probes (with or without PCR amplification) have a greater sensitivity for establishing the diagnosis of gonorrhoea and chlamydial infections.
See Molecular genetics - microbial.
McCormack WH and Rein MF. In: Mandell GR et al eds. Principles and Practice of Infectious Diseases. 6th ed. 2005. Churchill Livingstone.
McCormack WH. In: Mandell GR et al eds. Principles and Practice of Infectious Diseases. 6th ed. 2005. Churchill Livingstone.
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Its mission is to train and support pathologists and senior scientists and to improve the use of pathology testing to achieve better healthcare.
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