Keywords: Glucose-6-phosphate dehydrogenase quantitative, Glucose-6-phosphate dehyrogenase screen, G6PD
5 mL blood in ACD, lithium heparin or EDTA.
Semi-quantitative fluorescence test and methaemoglobin reduction test; enzyme assay.
Molecular genetics techniques are available - consult pathologist.
|Reference Interval:|| |
The G-6-PD assay reference intervals are:
3.0-4.2 IU/1010 red cells (WHO)
4.6-13.5 IU/g Hb.
Newborns and infants may have values that deviate from these values by 150%.
Investigation of haemolytic anaemia, particularly with features of oxidative haemolysis or clinical history suggestive of G-6-PD deficiency. Prior to commencing treatment with certain medications such as primaquine, tafenoquine and rasburicase.
The specific enzyme assay confirms deficiency.
Low levels indicate red cell G-6-PD deficiency.
Levels may be normal in acute haemolytic episodes due to reticulocytosis and, therefore, if deficiency is suspected, repeat G-6-PD testing may be indicated after resolution of haemolytic episode.
Beutler E. Blood 1994; 84(11): 3613-3636.
Bain et al. Dacie and Lewis Practical Haematology. 11th Ed. 2012.