Haemoglobin electrophoresis

Keywords: Haemoglobin EPP, Haemoglobin EPG, Hb EPP, Hb EPG, Haemoglobinopathy screen


5 mL blood in any anticoagulant.


Electrophoresis of a lysate of washed red cells; haemoglobins identified by mobility.

Reference Interval:

Refer to laboratory. Approximate ranges given below.

Normal adult: mainly HbA, small amounts HbA2 (1.5-3.5%), HbF (<1%).


Possible Thalassaemia or Haemoglobinopathy, including those haemoglobinopathies associated with DAT negative haemolysis, erythrocytosis, or cyanosis.


Haemoglobin variants with abnormal electrophoretic mobility will be detected eg, HbS, HbE. 

An interpretative comment will be provided by the pathologist.


Working Party of the General Haematology Task Force of the British Committee for Standards in Haematology. Br J Haematol 1998; 101: 783-792.

Bain et al. Dacie and Lewis Practical Haematology. 11th ed. 2012.

Ryan K et al. Significant haemoglobinopathies: guidelines for screening and diagnosis. BJH. 2010; 149; 35-49.

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