The main purpose of this Manual is to provide useful guidelines for the selection of pathology tests and to facilitate interpretation of results.
Contains a comprehensive listing of all genes from the Human Gene Nomenclature Committee (HGNC) database alongside laboratories and tests available in the country.
A manual for the process of macroscopic dissection in Anatomical Pathology laboratories.
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Keywords: Retics, Retic count
5 mL blood in EDTA tube.
Fresh blood incubated with:
supravital stain for ribosomal RNA and examined by microscopy; or
fluorescent dye taken up by RNA and cells counted by Flow cytometry.
Most current haematology analysers have reticulocyte modules to perform semi-automated or automated reticulocyte counts. Principle of test depends on the specific instrument.
The absolute reticulocyte count is calculated from the reticulocyte percentage and the red cell count.
Refer to laboratory. Term infant, cord blood: 2-6% (microscopy)
0.2-2.0% (10-100 x 109/L) (microscopy)
0.7-3.2% (30-140 x 109/L) (flow cytometry).
Indicated in the assessment of anaemia when bone marrow failure or haemolysis suspected;
apparent inadequate marrow response to haematinic therapy;
assessment of recovery after myelosuppression; and
monitoring haemolytic anaemia.
Reticulocyte count reflects marrow erythropoietic activity and corresponds to the finding of polychromasia on a blood film.
A low reticulocyte count in the presence of anaemia generally indicates bone marrow failure or haematinic deficiency.
Bain et al. Dacie and Lewis Practical Haematology. 11th Ed. 2012.
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The RCPA is the leading organisation representing Pathologists and Senior Scientists in Australasia.
Its mission is to train and support pathologists and senior scientists and to improve the use of pathology testing to achieve better healthcare.
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